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Discussion forum - 49th ERA-EDTA Congress
    Topic - Renal histopathology

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Poster: 416
Visits: 643
Title: CORRELATION OF INFLAMMATORY CELL INFILTRATION AND HISTOLOGIC AND CLINICAL MANIFESTATIONS OF LUPUS NEPHRITIS
Authors: Shiva Samavat , Pedram AhmadpoorPeyman TorbatiRamin GhaderiFatemeh Poorrezagholi Fariba Samadian Ahmad FiroozanMohsen Nafar
Centre: Labbafinejad Hospital, SBMU

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CORRELATION OF INFLAMMATORY CELL INFILTRATION AND HISTOLOGIC AND CLINICAL MANIFESTATIONS OF LUPUS NEPHRITIS

Shiva Samavat
Thread initiator
Subspeciality
Reg: 5/6/2012 12:42:00 PM

Comment# 1

I invite healthcare professionals to open debate around the issues raised in my poster, both those relating to research and to clinical practice.

Comment added on 5/6/2012 12:42:00 PM 
     

Meelad Habib

Subspeciality
Reg: 5/9/2012 9:53:00 PM

Comment# 2

 I would like to thank the authors for reporting the results of their study. 

I was wondering the following: 

(1) Why did the authors not stain for Th (CD4) and Tc (CD8) as important markers for T cells? 

(2) How did the researchers perform the analysis on the biopsies? Did they randomly choose fields in the biopsies or counted the whole tissue? 

 

Thanks!
 



Comment added on 12/3/2012 12:32:00 AM 
     

Shiva Samavat
Thread initiator
Subspeciality
Reg: 5/6/2012 12:42:00 PM



Meelad Habib wrote on 03/12/2012 0:32:00

 I would like to thank the authors for reporting the results of their study. 

I was wondering the following: 

(1) Why did the authors not stain for Th (CD4) and Tc (CD8) as important markers for T cells? 

(2) How did the researchers perform the analysis on the biopsies? Did they randomly choose fields in the biopsies or counted the whole tissue? 

 

Thanks!
 





Comment# 3

Dear Sir,

Thanks for the comment.

The answer to your question is as following:

". We did IHC staining for CD3 to identify lymphocytes as a whole, CD20 for B-cells and CD68 for macrophages.

Due to some technical and resource limitation, we did not stain for CD4 & CD8.

2. It was a retrospective study and was done on paraffine blocks, so all the remainnig tissue in the block was stained and counted. Patients with less than sufficeint biopsy material were excluded.

Thanks.



Comment added on 12/3/2012 1:10:00 PM 
Quoting Meelad Habib  

     

Meelad Habib

Subspeciality
Reg: 5/9/2012 9:53:00 PM

Comment# 4

 Thank you very much for your reply!



Comment added on 12/4/2012 6:34:00 PM 
     
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Most viewed poster for this congress
Poster: 424
Visits: 2539
Title: Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Glomerulonephritides: an Immunohystochemical expression analysis
Authors: Giuseppe Coppolino ,
Centre: Chair of Nephrology, University Magna Graecia of Catanzaro, Catanzaro, Italy


Poster most viewed in this topic
Poster: 424
Visits: 2539
Title: Neutrophil Gelatinase-Associated Lipocalin (NGAL) in Glomerulonephritides: an Immunohystochemical expression analysis
Authors: Giuseppe Coppolino ,
Centre: Chair of Nephrology, University Magna Graecia of Catanzaro, Catanzaro, Italy


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